Structure of the X (ADRP) domain of nsp3 from feline coronavirus
Identifieur interne : 002C94 ( Main/Exploration ); précédent : 002C93; suivant : 002C95Structure of the X (ADRP) domain of nsp3 from feline coronavirus
Auteurs : Justyna A. Wojdyla ; Ioannis Manolaridis ; Eric J. Snijder ; Alexander E. Gorbalenya ; Bruno Coutard ; Yvonne Piotrowski ; Rolf Hilgenfeld ; Paul A. TuckerSource :
- Acta Crystallographica Section D [ 1399-0047 ] ; 2009-12-01.
English descriptors
- Teeft :
- Acta, Acta cryst, Additional electron density, Adsc quantum, Ammonium, Ammonium citrate, Ammonium sulfate, Asymmetric unit, Binding cleft, Binding pocket, Binding site, Biol, Cell culture, Chloride ions, Citrate, Conformational changes, Coronaviral, Coronavirus, Coronaviruses, Cryst, Crystal diffracted, Crystal forms, Crystal structure, Cubic crystal form, Cubic space group, Cubic space groups, Data collection, Distinct properties, Domain, Egloff, Electron density, Embl hamburg, Enzymatic activity, Fcov, Fcov structure, Feline coronavirus, Gorbalenya, Ligand binding, Liquid nitrogen, Lysis, Lysis buffer, Macro, Macro domain, Macro domains, Molecular replacement, Molecule, Nature struct, Nsp3, Nsp3 acta cryst, Nucleic acids, Oscillation range, Other coronaviral, Other members, Peritonitis virus, Phosphatase activity, Preferred regions, Program coot, Program molprobity, Program refmac5, Protein residues, Protein sample, Ramachandran plot, Research papers, Ribbon representation, Search model, Side chain, Single crystals, Snijder, Sodium formate, Solvent content, Solvent molecules, Space group, Strain beaudette, Subgroup, Sulfate, Tetragonal, Tetragonal crystal form, Tetragonal space group, Uniprotkb, Uniprotkb reference, Viral, Virol, Wojdyla, Ziebuhr.
Abstract
The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol‐3‐phosphate was observed in the ADP‐ribose‐binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP‐ribose was detected in vitro. However, the structure with ADP‐ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro‐domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.
Url:
DOI: 10.1107/S0907444909040074
Affiliations:
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Le document en format XML
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<term>Adsc quantum</term>
<term>Ammonium</term>
<term>Ammonium citrate</term>
<term>Ammonium sulfate</term>
<term>Asymmetric unit</term>
<term>Binding cleft</term>
<term>Binding pocket</term>
<term>Binding site</term>
<term>Biol</term>
<term>Cell culture</term>
<term>Chloride ions</term>
<term>Citrate</term>
<term>Conformational changes</term>
<term>Coronaviral</term>
<term>Coronavirus</term>
<term>Coronaviruses</term>
<term>Cryst</term>
<term>Crystal diffracted</term>
<term>Crystal forms</term>
<term>Crystal structure</term>
<term>Cubic crystal form</term>
<term>Cubic space group</term>
<term>Cubic space groups</term>
<term>Data collection</term>
<term>Distinct properties</term>
<term>Domain</term>
<term>Egloff</term>
<term>Electron density</term>
<term>Embl hamburg</term>
<term>Enzymatic activity</term>
<term>Fcov</term>
<term>Fcov structure</term>
<term>Feline coronavirus</term>
<term>Gorbalenya</term>
<term>Ligand binding</term>
<term>Liquid nitrogen</term>
<term>Lysis</term>
<term>Lysis buffer</term>
<term>Macro</term>
<term>Macro domain</term>
<term>Macro domains</term>
<term>Molecular replacement</term>
<term>Molecule</term>
<term>Nature struct</term>
<term>Nsp3</term>
<term>Nsp3 acta cryst</term>
<term>Nucleic acids</term>
<term>Oscillation range</term>
<term>Other coronaviral</term>
<term>Other members</term>
<term>Peritonitis virus</term>
<term>Phosphatase activity</term>
<term>Preferred regions</term>
<term>Program coot</term>
<term>Program molprobity</term>
<term>Program refmac5</term>
<term>Protein residues</term>
<term>Protein sample</term>
<term>Ramachandran plot</term>
<term>Research papers</term>
<term>Ribbon representation</term>
<term>Search model</term>
<term>Side chain</term>
<term>Single crystals</term>
<term>Snijder</term>
<term>Sodium formate</term>
<term>Solvent content</term>
<term>Solvent molecules</term>
<term>Space group</term>
<term>Strain beaudette</term>
<term>Subgroup</term>
<term>Sulfate</term>
<term>Tetragonal</term>
<term>Tetragonal crystal form</term>
<term>Tetragonal space group</term>
<term>Uniprotkb</term>
<term>Uniprotkb reference</term>
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<front><div type="abstract" xml:lang="en">The structure of the X (or ADRP) domain of a pathogenic variant of feline coronavirus (FCoV) has been determined in tetragonal and cubic crystal forms to 3.1 and 2.2 Å resolution, respectively. In the tetragonal crystal form, glycerol‐3‐phosphate was observed in the ADP‐ribose‐binding site. Both crystal forms contained large solvent channels and had a solvent content of higher than 70%. Only very weak binding of this domain to ADP‐ribose was detected in vitro. However, the structure with ADP‐ribose bound was determined in the cubic crystal form at 3.9 Å resolution. The structure of the FCoV X domain had the expected macro‐domain fold and is the first structure of this domain from a coronavirus belonging to subgroup 1a.</div>
</front>
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<tree><noCountry><name sortKey="Coutard, Bruno" sort="Coutard, Bruno" uniqKey="Coutard B" first="Bruno" last="Coutard">Bruno Coutard</name>
<name sortKey="Gorbalenya, Alexander E" sort="Gorbalenya, Alexander E" uniqKey="Gorbalenya A" first="Alexander E." last="Gorbalenya">Alexander E. Gorbalenya</name>
<name sortKey="Hilgenfeld, Rolf" sort="Hilgenfeld, Rolf" uniqKey="Hilgenfeld R" first="Rolf" last="Hilgenfeld">Rolf Hilgenfeld</name>
<name sortKey="Manolaridis, Ioannis" sort="Manolaridis, Ioannis" uniqKey="Manolaridis I" first="Ioannis" last="Manolaridis">Ioannis Manolaridis</name>
<name sortKey="Piotrowski, Yvonne" sort="Piotrowski, Yvonne" uniqKey="Piotrowski Y" first="Yvonne" last="Piotrowski">Yvonne Piotrowski</name>
<name sortKey="Snijder, Eric J" sort="Snijder, Eric J" uniqKey="Snijder E" first="Eric J." last="Snijder">Eric J. Snijder</name>
<name sortKey="Tucker, Paul A" sort="Tucker, Paul A" uniqKey="Tucker P" first="Paul A." last="Tucker">Paul A. Tucker</name>
<name sortKey="Wojdyla, Justyna A" sort="Wojdyla, Justyna A" uniqKey="Wojdyla J" first="Justyna A." last="Wojdyla">Justyna A. Wojdyla</name>
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